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#1
12-14-2007, 06:45 AM
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Dispelling the cooked protein myth - denaturing is irrelevent
Probably one of the most asked questions I have seen is if cooking your proteins a certain way will render them worthless. After doing research over here in Champaign IL, I can say no, it won't.
In our lab, we literally replicate the human digestion process; and often use even harsher conditions. Proteins have the ability to form 3d structures through covalent and non-covalent attractions; these are secondary and tertiary structures (single peptide chain structures), and quaternary structures (interactions between more than one peptide chain). When protein enters the body, one of the first steps is the destruction of these 3d structures by pepsin. This is called denaturing. Then trypsin and HCL work to hydrolyze the the proteins into amino acids and di/tri-peptides which are absorbed by Pept 1 + individual amino transports, and the sodium potassium pump. The key point here is that all cooking your food is going to do is denature your proteins, and hydrolyze them into peptides / aminos. This will only assist in digestion! Let me just give you one step in our lab that should alleviate your concern: to completely hydrolyze the proteins into amino acids, we place our samples in 6 N of HCL and incubate them in an oven at 100 C for 24 hours! And they are still stable. You know why we use 6 N of HCL? Because that is what the body uses! Now go eat some denatured protein! [img]/forum/images/graemlins/tongue.gif[/img]
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Gabriel "Venom" Wilson, Ph.D. Nutritional Sciences B.S. (Hons) & M.S. in Kinesiology, CSCS Vice President, ABCbodybuilding Co-Editor. of JHR Venom@abcbodybuilding.com Bible Studies Click Here to Support the Future of Bodybuilding! Matthew 7:20 And Jesus said unto them, Because of your unbelief: for verily I say unto you, If ye have faith as a grain of mustard seed, ye shall say unto this mountain, Remove hence to yonder place; and it shall remove; and nothing shall be impossible unto you. 
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#2
12-18-2007, 05:53 AM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
Nice work boss man. How long did you get to test this? Great news for the well done steak guys in the world.
__________________
Lo, there do I see my father Lo, there do I see my mother and my sisters and my brothers Lo, there do I see the line of my people back to the beginning Lo, they do call to me They bid me take my place among them in the Halls of Valhalla Where the brave may live Forever. "Either you lift it or you don't." Jesse Dawson Marunde
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#3
12-18-2007, 11:34 AM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
This would apply to using whey protein in baking also?
__________________
The virtue of all achievement is victory over oneself. Those who know this can never know defeat. http://www.london2012.com/NR/rdonlyr...ghtlifting.jpg Creatine: A literature review Creatine: Practical Applications International Symposium of Biomechanics in Sport
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#4
12-18-2007, 09:10 PM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
Yup!
I have only been doing this since august; but its a standard protocol used by many scientists.
__________________
Gabriel "Venom" Wilson, Ph.D. Nutritional Sciences B.S. (Hons) & M.S. in Kinesiology, CSCS Vice President, ABCbodybuilding Co-Editor. of JHR Venom@abcbodybuilding.com Bible Studies Click Here to Support the Future of Bodybuilding! Matthew 7:20 And Jesus said unto them, Because of your unbelief: for verily I say unto you, If ye have faith as a grain of mustard seed, ye shall say unto this mountain, Remove hence to yonder place; and it shall remove; and nothing shall be impossible unto you.
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#5
12-21-2007, 10:11 PM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
What about freezing whey, Venom? Are there any "negative denaturing" effects there? It should be alright I would think.
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Feeding The Machine
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#6
12-22-2007, 03:33 PM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
Actually that decreases the degredative processes. Which is why we put our samples in our freezer between preparation steps.
__________________
Gabriel "Venom" Wilson, Ph.D. Nutritional Sciences B.S. (Hons) & M.S. in Kinesiology, CSCS Vice President, ABCbodybuilding Co-Editor. of JHR Venom@abcbodybuilding.com Bible Studies Click Here to Support the Future of Bodybuilding! Matthew 7:20 And Jesus said unto them, Because of your unbelief: for verily I say unto you, If ye have faith as a grain of mustard seed, ye shall say unto this mountain, Remove hence to yonder place; and it shall remove; and nothing shall be impossible unto you.
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#7
12-23-2007, 04:42 PM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
Good to know. I always though that myth sounded stupid. Boiled protein shake, anyone?
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#8
12-24-2007, 12:28 AM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
Would it be a good idea to freeze whey before use? Or is the normal consumption rate of a 5lb jug fast enough that it would not make a difference?
__________________
Lo, there do I see my father Lo, there do I see my mother and my sisters and my brothers Lo, there do I see the line of my people back to the beginning Lo, they do call to me They bid me take my place among them in the Halls of Valhalla Where the brave may live Forever. "Either you lift it or you don't." Jesse Dawson Marunde
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#9
12-24-2007, 02:00 AM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
No, you don't have to freeze it. I asked Layne Norton in my lab if it was necessary to freeze our samples after we hydrolyze the proteins w/ HCL and incubate them for 24 hrs. He made a great point: any damage that could be done was done at that point! Which appears to be minimal / none...so whether you freeze it or keep it out at room temperature should have little effect on the stability of the amino acids.
__________________
Gabriel "Venom" Wilson, Ph.D. Nutritional Sciences B.S. (Hons) & M.S. in Kinesiology, CSCS Vice President, ABCbodybuilding Co-Editor. of JHR Venom@abcbodybuilding.com Bible Studies Click Here to Support the Future of Bodybuilding! Matthew 7:20 And Jesus said unto them, Because of your unbelief: for verily I say unto you, If ye have faith as a grain of mustard seed, ye shall say unto this mountain, Remove hence to yonder place; and it shall remove; and nothing shall be impossible unto you.
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#10
12-27-2007, 01:58 AM
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Re: Dispelling the cooked protein myth - denaturing is irrelevent
My compliments, excellent explaination even us lamen can understand this one. [img]/forum/images/graemlins/smile.gif[/img]
__________________
rickck48-Life is one final lift to Perfection! John 15:5 I am the vine, ye are the branches; He that abideth in me, and I in him, the same bringeth forth much fruit; for with out me ye can do nothing. Goal: NOT there Yet, But haven't quit either! RANGERS LEAD THE WAY!
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