Dispelling the cooked protein myth - denaturing is irrelevent - ABCbodybuilding

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Old 12-14-2007, 07:45 AM
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Default Dispelling the cooked protein myth - denaturing is irrelevent

Probably one of the most asked questions I have seen is if cooking your proteins a certain way will render them worthless. After doing research over here in Champaign IL, I can say no, it won't.

In our lab, we literally replicate the human digestion process; and often use even harsher conditions.

Proteins have the ability to form 3d structures through covalent and non-covalent attractions; these are secondary and tertiary structures (single peptide chain structures), and quaternary structures (interactions between more than one peptide chain).

When protein enters the body, one of the first steps is the destruction of these 3d structures by pepsin. This is called denaturing. Then trypsin and HCL work to hydrolyze the the proteins into amino acids and di/tri-peptides which are absorbed by Pept 1 + individual amino transports, and the sodium potassium pump.

The key point here is that all cooking your food is going to do is denature your proteins, and hydrolyze them into peptides / aminos. This will only assist in digestion!

Let me just give you one step in our lab that should alleviate your concern: to completely hydrolyze the proteins into amino acids, we place our samples in 6 N of HCL and incubate them in an oven at 100 C for 24 hours! And they are still stable. You know why we use 6 N of HCL? Because that is what the body uses!

Now go eat some denatured protein! [img]/forum/images/graemlins/tongue.gif[/img]
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Old 12-18-2007, 06:53 AM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

Nice work boss man. How long did you get to test this? Great news for the well done steak guys in the world.
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Old 12-18-2007, 12:34 PM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

This would apply to using whey protein in baking also?
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Old 12-18-2007, 10:10 PM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

Yup!

I have only been doing this since august; but its a standard protocol used by many scientists.
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Matthew 7:20
And Jesus said unto them, Because of your unbelief: for verily I say unto you, If ye have faith as a grain of mustard seed, ye shall say unto this mountain, Remove hence to yonder place; and it shall remove; and nothing shall be impossible unto you.
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Old 12-21-2007, 11:11 PM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

What about freezing whey, Venom? Are there any "negative denaturing" effects there? It should be alright I would think.
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Old 12-22-2007, 04:33 PM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

Actually that decreases the degredative processes. Which is why we put our samples in our freezer between preparation steps.
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Matthew 7:20
And Jesus said unto them, Because of your unbelief: for verily I say unto you, If ye have faith as a grain of mustard seed, ye shall say unto this mountain, Remove hence to yonder place; and it shall remove; and nothing shall be impossible unto you.
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Old 12-23-2007, 05:42 PM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

Good to know. I always though that myth sounded stupid. Boiled protein shake, anyone?
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Old 12-24-2007, 01:28 AM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

Would it be a good idea to freeze whey before use? Or is the normal consumption rate of a 5lb jug fast enough that it would not make a difference?
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Old 12-24-2007, 03:00 AM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

No, you don't have to freeze it. I asked Layne Norton in my lab if it was necessary to freeze our samples after we hydrolyze the proteins w/ HCL and incubate them for 24 hrs. He made a great point: any damage that could be done was done at that point! Which appears to be minimal / none...so whether you freeze it or keep it out at room temperature should have little effect on the stability of the amino acids.
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Matthew 7:20
And Jesus said unto them, Because of your unbelief: for verily I say unto you, If ye have faith as a grain of mustard seed, ye shall say unto this mountain, Remove hence to yonder place; and it shall remove; and nothing shall be impossible unto you.
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Old 12-27-2007, 02:58 AM
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Default Re: Dispelling the cooked protein myth - denaturing is irrelevent

My compliments, excellent explaination even us lamen can understand this one. [img]/forum/images/graemlins/smile.gif[/img]
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